Table 1 Cryo-EM data collection, refinement, and validation statistics.
From: Structural Basis for pH-gating of the K+ channel TWIK1 at the selectivity filter
TWIK1 pH 7.4 EMDB 25168 PDB 7SK0 | TWIK1 pH 5.5 EMDB 25169 PDB 7SK1 | |
|---|---|---|
Data collection and processing | ||
Magnification | 36,000 x | 36,000 x |
Voltage (kV) | 200 | 200 |
Micrographs (no.) | 5014 | 3217 |
Electron exposure (e–/Å2) | 49.715 | 50.3523 |
Defocus range (μm) | −0.6 to −2.0 | −0.6 to −2.0 |
Super-resolution pixel size (Å) | 0.5685 | 0.5575 |
Map pixel size (Å) | 1.137 | 1.115 |
Symmetry imposed | C2 | C2 |
Topaz particle images (no.) | 2,872,868 | 8,990,305 |
Final particle images (no.) | 92,438 | 38,126 |
Map resolution (Å) | 3.4 | 3.4 |
FSC threshold | 0.143 | 0.143 |
Refinement | ||
Model resolution (Å) | 3.4 | 3.4 |
FSC threshold | 0.143 | 0.143 |
Map sharpening B factor (Å2) | −112.7 | −111.91 |
Model composition | ||
Nonhydrogen atoms | 4,153 | 4,297 |
Protein residues | 514 | 524 |
Ligands | 7 | 7 |
B factors (Å2) | ||
Protein | 147.3 | 143.13 |
Ligand | 78.45 | 108.96 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.007 | 0.008 |
Bond angles (°) | 1.101 | 1.067 |
Validation | ||
MolProbity score | 1.14 | 0.99 |
Clashscore | 1.2 | 0.7 |
Poor rotamers (%) | 0 | 0.43 |
Ramachandran plot | ||
Favored (%) | 95.69 | 96.15 |
Allowed (%) | 4.31 | 3.85 |
Disallowed (%) | 0 | 0 |
