Fig. 2: Metabolic rewiring supporting growth rate optimization in the ETS variants.
a Transcriptional and metabolic flux distribution correlations between evolved replicates and unevolved strains. Correlation of all four evolved replicates of each ETS variant has been calculated with corresponding unevolved strain and has been presented as Pre-vs.-Post ALE strains correlation. Correlation of all four evolved replicates of each ETS variant has been calculated among each other and has been presented as Endpoint Replicates correlation. b Expression changes in the alternate NqRED and O2RED in the unbranched ETS variants. c Computed metabolic flux maps depicting the central metabolism in the evolved ETS variants as compared to respective unevolved ETS variants. Key metabolites are indicated in the figure as follows: glc glucose, g6p D-glucose-6-phosphate, f6p D-fructose-6-phosphate, 6 pg 6-phospho-D-gluconate, ru5p D-ribulose-5-phosphate, r5p ɑ-D-ribose 5-phosphate, xu5p D-xylulose-5-phosphate, s7p sedoheptulose-7-phosphate, e4p D-erythrose-4-phosphate, dha dihydroxyacetone, dhap dihydroxyacetone-phosphate, kdpg 2-keto-3-deoxy-6-phosphogluconate, g3p glyceraldehyde-3-phosphate, pep phosphoenolpyruvate, pyr pyruvate, ac acetate, accoa acetyl-CoA, oaa oxaloacetate, cit citrate, acon cis-aconitate, icit isocitrate, akg 2-oxoglutarate, succoa succinyl-CoA, succ succinate, fum fumarate, mal malate. [oxPPP oxidative pentose phosphate pathway, EMP Embden-Meyerhof-Parnas pathway, ED Entner–Doudoroff pathway, TCA Tricarboxylic acid cycle]. Source Data available in Supplementary Table 2 and provided RNA-seq data.
