Fig. 1: LECT2 is a positive regulator of RIG-I-mediated innate immune responses.

a qRT-PCR analysis of LECT2 mRNA in HepG2-WT and HepG2-LECT2 cells. Results were normalised to those of ACTB. b qRT-PCR analysis of IFNB1 mRNA in HepG2-WT and HepG2-LECT2 cells at 12 h after mock (Cont) or HCV-RNA transfection. Data shown are means of 3 technical replicates ± SEM. p = 0.001 by two-way ANOVA with Tukey’s multiple comparison test. c Immunoblot analysis of RIG-I, MDA5, p-IRF3, IRF3, LECT2 and β-actin in HepG2-WT and HepG2-LECT2 cells at 6 h after mock (Cont) or HCV-RNA transfection. d qRT-PCR analysis of IFNB1, MX1 and OAS2 in non-treated (Nont) and r-LECT2-treated HepG2 cells at 0, 3, 6 and 12 h after HCV-RNA transfection. Data shown are means of 2 technical replicates. e qRT-PCR analysis of IFNB1, MX1 and OAS2 in non-treated (Nont), proteinase-treated r-LECT2 (r-LECT2 + proteinase), and r-LECT2-treated cells (Cont siRNA-transfected HepG2 cells, RIG-I siRNA-transfected HepG2 cells, and MDA5 siRNA-transfected HepG2 cells) at 12 h after HCV-RNA transfection. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001, not significant (ns) by two-way ANOVA with Tukey’s multiple comparison test. f Immunoblot analysis of RIG-I, MDA5, and β-actin in Nont, r-LECT2 + proteinase and r-LECT2-treated cells at 12 h after HCV-RNA transfection. g 50 ng/mL r-LECT2 and LECT2 specific antibody (0, 50 and 100 ng/mL) were reacted for 15 min at room temperature, and then their reactants were treated with HepG2 cells for 1 h and then transfected with HCV-RNA. (upper) qRT-PCR analysis of IFNB1 at 12 h after HCV-RNA transfection. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001 by two-way ANOVA with Tukey’s multiple comparison test. (lower) Immunoblot analysis of RIG-I, MDA5, p-IRF3, IRF3, LECT2 and β-actin at 12 h after HCV-RNA transfection. Source data are provided as a Source data file.