Fig. 3: MET has an important role in innate immune responses.

a Immunoblot analysis of MET and β-actin in HepG2-WT and HepG2-MET-KO cells at 12 h after Cont or Poly(I:C) transfection. b qRT-PCR analysis of IFNB1 in HepG2-WT and HepG2-MET-KO cells at 12 h after Cont or Poly(I:C) transfection. Results were normalised to those of ACTB. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001 by two-way ANOVA with Tukey’s multiple comparison test. c, d C57BL/6J mice were injected intravenously with Cont Gapmer or Met Gapmer, and at 1 day after injection, the mice were infected intraperitoneally with 1.0 × 10⁴ pfu LCMV. c Immunoblot analysis of MET and β-actin in the liver of WT mice at 5 days after LCMV infection. d qRT-PCR analysis of LCMV, Ifnb1 and Ddx58 in the liver of WT mice at 5 days after LCMV infection. Data shown are means of 5 biological replicates ± SEM. ***p < 0.001 by two-way ANOVA with Tukey’s multiple comparison test. Boxplots: centre line, median; box limits, 25 to 75th percentiles; whiskers, min to max. e Immunoblot analysis of p-MET, MET, p-GAB1, GAB1, p-SHP2, SHP2 and β-actin in non-treated HepG2 cells and HGF-treated HepG2 cells at 12 h after Cont or Poly(I:C) transfection. f qRT-PCR analysis of IFNB1 in non-treated HepG2 cells and HGF-treated HepG2 cells at 12 h after Cont or Poly(I:C) transfection. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001 by two-way ANOVA with Tukey’s multiple comparison test. g HepG2-RIG-I-KO cells were transfected with RIG-I plasmid, HA-ubiquitin plasmid. At 48 h after transfection, the cells were treated with HGF (0, 5 and 10 ng/mL) for 1 h, then transfected with Poly(I:C) in the presence of MG132. At 12 h after Poly(I:C) transfection, lysates were immunoprecipitated with an anti-RIG-I antibody. Immunoblot analysis of K48-Ub, RIG-I, p-MET, MET, p-SHP2, SHP2 and β-actin in immunoprecipitated samples and input cell lysate. Source data are provided as a Source data file.