Fig. 7: PTP4A1 is essential for the enhancement of innate immune response by LECT2.

a Immunoblot analysis of MET, PTP4A1, p-SHP2, RIG-I and β-actin in immunoprecipitated samples and input cell lysate. b HepG2-MET-KO cells were transfected with Control, MET and PTP4A1 plasmid or co-transfected with MET and PTP4A1 plasmid. These cells were treated with 50 ng/mL r-LECT2 for 1 h and then transfected with HCV-RNA. qRT-PCR analysis of IFNB1 in these cells. Results were normalised to those of ACTB. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001, not significant (ns) by two-way ANOVA with Tukey’s multiple comparison test. c–e KH-MET-KO cells were transfected with Control, MET and PTP4A1 plasmid or co-transfected with MET and PTP4A1 plasmid. These cells were treated with 50 ng/mL r-LECT2 for 1 h and then transfected with H77S.3/GLuc-RNA. c Immunoblot analysis of MET, PTP4A1 and β-actin in these cells. d qRT-PCR analysis of IFNB1, DDX58 and IFIH1 in these cells. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001, **p = 0.002, not significant (ns) by two-way ANOVA with Tukey’s multiple comparison test. e GLuc activity in these cells at 48 h after H77S.3/GLuc-RNA transfection. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001, **p = 0.002, not significant (ns) by two-way ANOVA with Tukey’s multiple comparison test. f (left) Co-immunofluorescence staining of MET and PTP4A1 in HepG2 cells. DAPI was used to stain nuclei. f (right) Co-localization was estimated using Pearson’s correlation coefficient and Manders’ co-localization coefficients M1 (red per green) and M2 (green per red). Boxplots: centre line, median; box limits, 25 to 75th percentiles; whiskers, min to max. g (left) Immunoblot analysis of PTP4A1, RIG-I and β-actin at 12 h after HCV-RNA transfection. g (right) qRT-PCR analysis of IFNB1 in HepG2-WT and HepG2-PTP4A1-KO cells at 12 h after HCV-RNA transfection. Data shown are means of 3 technical replicates ± SEM. ***p < 0.001, not significant (ns) by two-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source data file.