Fig. 3: Differential regulation of the RAS-Myc axis by melanomagenic and non-melanomagenic NRAS mutants.
From: Enhanced BRAF engagement by NRAS mutants capable of promoting melanoma initiation
a Dot plots representing the molecular functions subset of Gene Ontology (GO) analysis of genes upregulated (left) or downregulated (right) in TN61R/R and TN61H/H MEFs compared to TN61P/P MEFs. Three biological replicates per genotype were used for analyzed. Bar plot showing the differential enrichment of Hallmark gene sets (p-adjusted < 0.05) in MEFs expressing NRAS61R/R versus NRAS61P/P (b) or NRAS61H/H versus NRAS61P/P (c). d Dot plot of flow cytometric analysis of EdU labeling in NRAS-mutant MEFs. n = 4 biologically independent MEF lines per genotype were examined over 4 independent experiments. e Representative image of EdU (proliferation, green) and gp100 (melanocyte, red) co-staining in skin harvested from a ten-day old mouse. n = 4 biologically independent mice were examined per genotype. f Dot plot of percent EdU positivity in melanocytes from 10-day old TN61X/X mouse skin. The following number biologically independent animals were evaluated per genotype (K/K = 5, L/L = 4, H/H = 4, P/P = 3). Dot plot data are presented as mean values +/− SD where each dot represents one biological replicate. One-way ANOVA with a Tukey’s post-test was used to compare data between each genotype. NRAS mutant samples statistically different from NRAS61R/R samples are indicated in the figure. Adjusted p-values for all comparisons can be found in Supplementary Table 2. * p < 0.05, † p < 0.001. Source data are provided as a Source Data file.
