Fig. 6: Melanomagenic NRAS mutants enhance RAF dimerization.
From: Enhanced BRAF engagement by NRAS mutants capable of promoting melanoma initiation
a Schematic representation of the RAF NanoBiT assay in which each RAF isoform is tagged with either LgBiT or SmBiT. Dot plots of normalized luminescence intensity in TN61X/X MEFs infected with adenovirus expressing BRAF-LgBiT and BRAF-SmBiT (b), BRAF-LgBiT and Craf-SmBiT (c), CRAF-LgBiT and CRAF-SmBiT (d), ARAF-LgBiT and BRAF-SmBiT (e), ARAF-LgBiT and ARAF-SmBiT (f), or ARAF-LgBiT and CRAF-SmBiT (g). Luminescence intensity was normalized to crystal violet staining for each well. Dot plot data are presented as mean values +/− SD where each dot represents one biological replicate. The following biologically independent replicates per genotype were examined over five independent experiments (BRAF-BRAF: Q/Q = 5, R/R = 5, H/H = 4; P/P = 4; BRAF-CRAF: Q/Q = 5, R/R = 5, H/H = 4; P/P = 5; CRAF-CRAF: Q/Q = 5, R/R = 5, H/H = 4; P/P = 4; BRAF-ARAF: Q/Q = 4, R/R = 4, H/H = 4; P/P = 4; ARAF-ARAF: Q/Q = 4, R/R = 4, H/H = 4; P/P = 4; ARAF-CRAF: Q/Q = 4, R/R = 4, H/H = 4; P/P = 4). One-way ANOVA with a Tukey’s post-test was used to compare data between each genotype. NRAS mutant samples statistically different from NRAS61R/R samples are indicated in the figure. Adjusted p-values for all comparisons can be found in Supplementary Table 4d. * p < 0.05, ** p < 0.01, † p < 0.001, ‡ p < 0.0001. Source data are provided as a Source Data file.
