Fig. 2: Adult salivary gland organoids possessed distinct gland-specific properties in mice.

a–d mPG (top) or mSLG (bottom) organoids were maintained in the GEM for 9 days, followed by 3 days of differentiation in the DAM. a Representative images of brightfield microscopy (left) and H&E staining (right). Scale bars indicate 100 μm. b The presence of mucin in mPG and mSLG tissues (left) or organoids (right) was assessed via PAS staining. Black arrows indicate the mucin-positive region near end buds. Scale bars indicate 50 μm. c IF staining for PG-specific (AMY2, green) and SLG-specific (MUC19, red) markers in tissues (left) or organoids (right). Nuclei were stained with Hoechst 33342 (blue). Scale bars indicate 50 μm. d PG-specific Amy2 or SLG-specific Muc19 gene expressions were validated via qRT-PCR in organoids. Data were presented as the mean ± SEM (n = 3 biologically independent samples). e Transmission electron microscopy (TEM) images of mPG (left), and mSLG (right) organoids. Red asterisks indicate internal lumen. Red arrows indicate serous acinar cells or mucous acinar cells in mPG or mSLG organoids, respectively. Scale bars indicate 10 μm. f–h Murine salivary gland organoids were maintained in the GEM for 9 days, followed by 3 days of differentiation in the DAM, and subjected to RNAseq analysis (n = 2 biologically independent samples). f DEGs with a fold change >2 and p-value <0.05 between mPG (green) and mSLG (red) organoids depicted using a volcano plot. g DEGs with a fold change >2 and p-value <0.05 between mPG (green) and mSMG (blue) organoids depicted using a volcano plot. h DEGs with a fold change >2 and p-value <0.05 between mSLG (red) and mSMG (blue) organoids depicted using a volcano plot. **p < 0.01, ***p < 0.001. Source Data are provided as a source data file.