Fig. 5: Single-cell RNA-seq revealed cellular heterogeneity and glandular diversity in human adult salivary gland organoids. | Nature Communications

Fig. 5: Single-cell RNA-seq revealed cellular heterogeneity and glandular diversity in human adult salivary gland organoids.

From: Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands

Fig. 5

a–e Human PG, SMG, and SLG organoids cultured for 1 month in the GEM, followed by differentiation in the DAM for another 3 days, and subjected to single-cell RNA sequencing. Data from three gland organoids were analyzed and shown together. a Cell clusters were visualized as uniform manifold approximation and projection (UMAP) for human salivary gland organoids (n = 10,287). b The expression of cluster 1 (basal)-specific genes (BNC1, KRT5, and KRT15) was visualized in UMAPs for salivary gland organoids (top) and tissues (bottom). c Acinar cell (SPDEF, AQP5, and BPIFA2) and luminal duct cell (LCN2 and PIGR) markers were enriched in cluster 3, as visualized in UMAPs for salivary gland organoids (top) and tissues (bottom). d Acinar cell markers (HTN1, DNER, CHRM3) were enriched in cluster 4, as visualized in UMAPs for salivary gland organoids (top) and tissues (bottom). e Pseudotime analysis displayed inferred divergent trajectory into cycling (downward to cluster 2) or differentiation (rightward to cluster 3) stages from cluster 1 (basal) in organoids. f DEGs of PG (left), SMG (middle), or SLG (right) from both organoids and tissues displayed based on scRNA-seq data. The size of the circle represents the percentage of a cell population, while its color depicts gene expression.

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