Fig. 7: TRPM7 is activated by acidic pH in macrophages.
From: Efferocytosis requires periphagosomal Ca2+-signaling and TRPM7-mediated electrical activity

a Flow cytometry-based measurement of phagocytosis in BMDMs treated with media or FTY720 (5 μM) during phagocytosis of apoptotic Jurkat cells (J:B = 10:1). Left panel shows cargo association with BMDMs (CellTrace Violet+ CypHer5E+). Right panel shows acidification of engulfed cargo (Cypher5E MFI). Data points are mean of n = 3 independent samples; error bars are SD. Results are typical of two independent experiments. b Schematic of perforated-patch configuration used for electrophysiology. c Perforated-patch electrophysiology of ITRPM7 in BMDMs in response to extracellular pH. Left: Time-current relationship of ITRPM7 activation by bath pH as revealed in the perforated-patch recording. Middle: Representative I–V relationship of ITRPM7 and block by FTY720 at pH 4.0. Right: Quantification of current densities at −100 mV (inward current) and +100 mV (outward current). Dots represent individual cell recordings with quantification under each condition shown. Error bars are SEM. d pH-dependent activation of ITRPM7 in WT BMDMs using perforated-patch electrophysiology. Left: Time-current relationship of ITRPM7 activation and block by 10 mM MgCl2. Right: Representative I–V relationship of perforated-patch-clamp ITRPM7 current in response to varying bath conditions. e pH-dependent activation of ITRPM7 in KO BMDMs using perforated-patch electrophysiology. Left and Right panels are as described in panel d. Source data are provided as a Source Data file, and statistical testing is described in “Statistics and Reproducibility”.