Fig. 2: Enriched expression of circRNAs in inhibitory and excitatory neurons.
From: Exploring the cellular landscape of circular RNAs using full-length single-cell RNA sequencing
a The t-SNE plot of all 41,911 brain single cells, colored by annotated cell types. GABA, GABAergic neurons. GLUT, glutamatergic neurons. MG, microglia cells. b Panels from left to right: the number of cells; the number of identified circRNAs; expression values of top 10 highly expressed circRNAs; expressed values of cell-type-specific circRNAs in each cluster. Sizes of points indicate the mean expression values measured by CPM. c The cellular specificity of circRNAs (n = 74,678), circRNA hosting genes (n = 13,467), and non-hosting genes (n = 39,253). Upper, the cell specificity was measured by the Tau method. Bottom, the proportion of cell types in all expressing cells. d Example of 12 circRNAs generated from the Taf1 loci. Upper, schematic view of gene structure and back-splicing events (grey lines). Bottom, mean expression levels of circRNA isoforms and Taf1 gene in each cell cluster. e Orthologues between human and mouse circRNAs. All human and circRNAs were ranked according to their expression levels, and the y axis represents the average CPM of circRNAs in all expressing cells. Red, circRNAs that are conservatively expressed in human and mouse. Grey, circRNAs uniquely detected in each species. f Expression heatmap of 1,048 orthologous circRNAs in human and mouse cells. Filled colors represent the mean CPM of circRNAs in each cluster. g The Spearman correlation between circRNAs and host genes (n = 13,467) or RBPs (n = 2995). h Cell-type-specific expression of RBPs and circRNAs. Filled colors indicate the normalized value of gene or circRNA expression values in each cell type. All center lines in the box plots and violin plots indicate the median values, and box limits indicate the upper and lower quartiles of plotted values. The upper and lower whiskers indicate the largest and smallest values within the range of 1.5x IQR from the box limits. ***P < 0.001, Wilcoxon rank-sum test (two-sided). Source data are provided as a Source Data file.
