Fig. 6: Effective strategies for inducing silk gland cell growth.
From: A single-cell transcriptomic atlas characterizes the silk-producing organ in the silkworm
a Immunostaining of silk gland cells dissected from E8D, 1L1D, and 1LM individuals. Nuclei were stained with DAPI (white); the cytoskeleton was stained with phalloidin (red). The scale bar represents 10 µm. Representative images are chosen from photos of at least 10 intact silk glands. b The cross-sectional area of ASG/MSG/PSG cells was calculated from E8D to 1LM. Data are presented as mean ± SD (n = 8). Source data are provided as a Source Data file. c Immunofluorescence staining for Btl in 1L1D individuals. The spatial expression pattern is shown in red. The white arrowhead indicates the boundary between the ASG and MSG; the white arrow indicates the boundary between the MSG and PSG (n ≥ 10 in three independent experiments). d–f Enriched GO/KEGG terms (p < 0.05) related to cell growth (d), communication (e) and renewal (f), p value was calculated by the hypergeometric distribution, p-adj was obtained after p value is corrected by Benjamin & Hochberg multiple test. Color gradients indicate the range of p values. The p-values of each pathway are provided in Supplementary Data 12. ASG anterior silk gland, MSG middle silk gland, PSG posterior silk gland. E8D, 8 days post egg laying, 1L1D day 1 of the first instar, 1LM first larval molting.
