Fig. 1: Schematic of vaccination schedule and sample collection.
From: Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine
All participants were vaccinated with BNT162b2 on days 0 and 21 and samples were collected early in 2021. A 10 healthy participants underwent serial phlebotomy that was performed pre-vaccination (day −3 to 0), on day 28–30, and on day 105-108. PBMCs were isolated at each time point, and citrated plasma was stored when possible. PBMCs from these participants were utilized for CyTOF and in vitro stimulation studies. Plasma was used both for SARS-CoV-2 ELISAs and vesicular stomatitis virus pseudoneutralization assays. B A single healthy participant underwent serial phlebotomy pre-vaccination and on days 8, 14, 28, and 42. PBMCs and citrated plasma were isolated at each time point and used for transcriptional analysis of SARS-CoV-2-specific B cells.
