Fig. 7: BNT162b2 vaccination drives IgG and IgA-switched SARS-CoV-2-binding memory B-cell and plasmablast expansion.
From: Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine
B cells that bound a panel of LIBRA-seq antigens were purified from peripheral blood samples and single-cell RNA-seq/BCR-seq/LIBRA-seq data were integrated. Cells were scored positive for binding a given antigen for LIBRA-seq scores ≥1. A UMAP identified clusters of B cells based on transcriptional similarity. Antibody-secreting cells (pink circle encompassing clusters 7 and 9) and isotype-switched memory B cells (blue circle encompassing clusters 0, 2, 5, and 6) are highlighted across all UMAP plots. B BCR isotypes are shown among sorted B cells. C Selected gene expression profiles within each cluster are shown. D–F SARS-CoV-2-binding B cells were identified using LIBRA-seq and non-class-switched B cells were filtered out for detailed analysis. D UMAP identified clusters of SARS-CoV-2-binding B cells based on transcriptional similarity. E IgH somatic hypermutation frequency of SARS-CoV-2-binding B cells is indicated. F SARS-CoV-2-binding B-cell isotypes are shown for each time point. G SARS-CoV-2-binding B cells are shown, each panel indicates cells that also bound the indicated coronavirus S antigens.
