Fig. 2: T3 expands adipocyte progenitor population in iBAT depot. | Nature Communications

Fig. 2: T3 expands adipocyte progenitor population in iBAT depot.

From: Triiodothyronine (T3) promotes brown fat hyperplasia via thyroid hormone receptor α mediated adipocyte progenitor cell proliferation

Fig. 2

a–f Number of adipocyte progenitor cells (APCs) in iBAT per mouse (a, d) and percentage of APCs in live SVF cells of iBAT (b, e) in MMI mice and MMI mice with daily T3 injection for 4 d at indicated doses (n = 6) or for indicated days at 0.5X standard dose (n = 5). FACS plot of the APCs (c, f). Cells were pre-gated on live CD45− cells. g Schematic of EdU labeling experiment. h Immunofluorescence staining of UCP1 and Hoechst with EdU labeling on iBAT sections. Scale bar, 20 μm. i–k Number of BrdU+ APCs in iBAT per mouse (i), FACS plot and quantification of BrdU+ APCs in iBAT (j), and mean fluorescence intensity (MFI) of Ki67 staining in APCs of iBAT (k) in MMI mice and MMI mice with daily T3 injection for indicated days at 0.5X standard dose (n = 5). Cells were pre-gated on live CD45−CD31−Sca1+ cells. l BrdU incorporation analysis of brown adipocyte precursor cell line (BAC) cultured in TH-deficient (TD) medium after T3 treatment (n = 3). Means ± SEM are shown. P values were calculated by two-tailed unpaired Student’s t test for a, b, d, e, i-l. NS, not significant. Source data are provided as a Source Data file.

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