Fig. 4: Deletion of TRβ by Myf5-cre or TRα by UCP1-Cre barely affects APC proliferation in iBAT.

a Breeding scheme for generating MTRβKO mice. b Relative TRβ mRNA levels in iBAT depots of TRβFloxed (Floxed) and MTRβKO mice (n = 5). c Representative photograph (left) and FW of iBAT (right) of Floxed and MTRβKO mice with daily PBS or 0.5X standard dose of T3 injection for 5 d (n = 5). d, e Number of APCs in iBAT per mouse (d, n = 5) and percentage of APCs in live SVF cells of iBAT (e, n = 5) from Floxed and MTRβKO mice with daily PBS or 0.5X standard dose of T3 injection for 5 d. f MFI of Ki67 staining in APCs of Floxed and MTRβKO mice with daily PBS or 0.5X standard dose of T3 injection for 5 d (n = 5). g FACS plot and quantification of the BrdU⁺ APCs in iBAT from Floxed and MTRβKO mice with daily PBS or 0.5X standard dose of T3 injection for 5 d (n = 5). Cells were pre-gated on live CD45⁻CD31⁻Sca1⁺ cells. h Breeding scheme for generating UTRαKO mice. i Relative TRα mRNA levels in iBAT depots of TRαFloxed (Floxed) (n = 7) and UTRαKO mice (n = 6). j, k Number of APCs in iBAT per mouse (j) and percentage of APCs in live SVF cells of iBAT (k) from Floxed and UTRαKO mice with daily PBS (n = 3) or 0.5X standard dose of T3 (n = 5) injection for 5 d. l FACS plot and quantification of the BrdU⁺ APCs in iBAT from Floxed and UTRαKO mice with daily T3 injection at 0.5X standard dose for 5 d (n = 5). Cells were pre-gated on live CD45⁻CD31⁻Sca1⁺ cells. Means ± SEM are shown. P values were calculated by two-tailed unpaired Student’s t test for b–g, i–l. NS, not significant. Source data are provided as a Source Data file.