Fig. 9: Knockdown of Myc or Hk2 attenuates the T3 effect on cell state transition and cell cycle progression.

a qRT-PCR analysis of Myc or Hk2 in APCs from iBAT of shRNA-injected mice (n = 3). b qRT-PCR analysis of selected Group 1-specific genes and Group 2-specific genes in APCs from iBAT of shRNA-injected mice with daily T3 injection for 4 d (n = 3). c Percentage of Dpp4⁺ APCs, Icam1⁺ APCs in iBAT of shRNA-injected mice with daily T3 injection for 4 d (n = 3). d Violin plots showing the expression levels and distribution of Ccnb1, split by “orig.ident” (MMI group and T3 group). e Comparison of the percentage of Ccnb1 positive cells in Group 5 cells between MMI group and T3 group. f Percentage of BrdU⁺ APCs in iBAT of shRNA-injected mice with daily T3 injection for 4 d (n = 3). g FACS plot of the BrdU⁺ APCs in iBAT of shRNA-injected mice with daily T3 injection for 4 d (n = 3). Cells were pre-gated on live CD45⁻CD31⁻Sca1⁺ cells. h, i qRT-PCR analysis of Group 5 markers (Stmn1 and Cenpa) (h) and G2/M phases marker (Ccnb1) (i) in APCs from iBAT of shRNA-injected mice with daily T3 injection for 4 d (n = 3). Means ± SEM are shown. P values were calculated by two-tailed unpaired Student’s t test for a–c, f, h, and i. NS, not significant. Source data are provided as a Source Data file.