Fig. 5: MRE11 resects G4 telomere. | Nature Communications

Fig. 5: MRE11 resects G4 telomere.

From: Dynamic interaction of BRCA2 with telomeric G-quadruplexes underlies telomere replication homeostasis

Fig. 5

a Schematic representation of human MRE11. The functional domains are marked. Recombinant hMRE11 core (1–411 a.a.; MRE11N) was purified, following the previously published method62 (SDS-PAGE gel on the right). Functionality of the purified protein was assessed (Supplementary Fig. 14) and employed in the nuclease assay. b, c, d, e Denaturing PAGE gels showing nuclease activity of MRE11N (lane 2-3) and the effect of BRCA2OB towards various telomere G4 and G3 variants (lane 4-5). 2.5 μM or 6 μM of MRE11N was incubated with indicated DNA substrates: b TelG5-G3; c TelG5; d TelG5TTT; e TelG3GGG for nuclease assay. Increasing concentrations of BRCA2OB were employed to assess the inhibition of MRE11-mediated resection. The result is the representative of three independent experiments.

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