Fig. 6: Therapeutic efficacy of ECN-pE(C/A)2 against DSS-induced murine IBD with delayed treatment. | Nature Communications

Fig. 6: Therapeutic efficacy of ECN-pE(C/A)2 against DSS-induced murine IBD with delayed treatment.

From: Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery

Fig. 6

a Experimental procedure for the treatment of DSS-induced murine IBD. C57BL/6 mice were given drinking water containing 3% DSS from day 0 to day 5. Then, the mice were fed PBS, ECN(C/A)2, ECN-pE(C/A)2, or ECN-pE (1 × 108 CFU) on days 5, 6, 7, and 9. b The body weight of mice during the treatment. c The DAI of mice during the treatment. d Photographs and (e) corresponding quantified lengths of colons harvested from mice after different treatments on day 10. Scale bar: 2 cm. f Representative H&E staining images of colon tissues harvested on day 10 after different treatments from five biologically independent animals in each group. Scale bar: 200 μm. g The MPO activity in the colon of mice after treatment. MPO myeloperoxidase. h–l The levels of IL-1β, TNF-α, IL-6, IL-10, and TGF-β in the colon tissues measured by ELISA on day 10 with delayed treatment. Data are presented as mean values ± SEM (n = 5 biologically independent samples for (b, c, e and g–l)). Statistical analysis was evaluated with two-tailed Student’s t tests (*P < 0.05, **P  < 0.01, ***P  <  0.001 and ****P<0.0001). DSS dextran sodium sulfate. Source data are provided as a Source Data file.

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