Fig. 3: Inducible enhancer activation is linked to increased eRNA burst fractions.
From: Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters
a Inducible enhancers associated with inducible genes in macrophages with GRO-seq reads from resting and 1 h LPS-activated macrophages, ChIP-seq for PU.1 and STAT2, IRF3 and H3K27ac. Macrophage enhancers were defined previously31. The positions of smRNA-FISH probes are magenta. b smRNA-FISH Images of eRNA bursts in untreated and LPS-activated macrophages with probes for the Egr2 enhancer (0 and 60 min LPS), the Il12b HSS1 and Ifnb1 L2 (0 and 90 min LPS), and the Peli1 enhancer (0 and 120 min LPS). smRNA-FISH probes are in magenta. At least three independent biological replicates per gene and condition, n = 64,682 cells. c eRNA burst fractions in resting and LPS-activated macrophages. At least three independent biological replicates per gene and condition, n = 64682 cells. d eRNA burst intensities in resting and LPS-activated macrophages. P values were determined by a two-sided Tukey HSD test (ANOVA design formula ∼ Sample + Replicate) and adjusted for multiple testing. At least three independent biological replicates per gene and condition, n = 64,682 cells.
