Fig. 8: Similar enhancer–promoter distances in wild-type and cohesin-deficient macrophages.
From: Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters
Cumulative distances between enhancer and promoter smRNA-FISH signals in LPS-activated macrophages at the indicated loci. Number of wild-type cells: 8382 (Egr2), 3985 (Prdm1), 16,604 (Il12b), 19,864 (Peli1), at least three biological replicates per locus. Number of Rad21−/− cells: 7658 (Egr2), 3992 (Prdm1), 7352 (Il12b), 18,600 for Peli1), at least three biological replicates per locus. Top: all observed distances. Number of wild-type gene-enhancer pairs: 1754 (Egr2), 59 (Prdm1), 370 (Il12b), 5004 (Peli1). Number of Rad21−/− gene-enhancer pairs: 128 (Egr2), 35 (Prdm1), 43 (Il12b), 4288 (Peli1). Bottom: Distances up to 1 μm. Number of wild-type gene-enhancer pairs: 534 (Egr2), 23 (Prdm1), 116 (Il12b), 1440 (Peli1). Number of Rad21−/− gene-enhancer pairs: 52 (Egr2), 20 (Prdm1), 19 (Il12b), 956 (Peli1). P values < 0.05 indicate that two cumulative distributions are different by the two-sided Kolmogorov–Smirnov test.
