Fig. 2: Matriglycan microarrays of defined lengths for binding studies. | Nature Communications

Fig. 2: Matriglycan microarrays of defined lengths for binding studies.

From: Cell surface glycan engineering reveals that matriglycan alone can recapitulate dystroglycan binding and function

Fig. 2

a Chemoenzymatic synthesis scheme of defined lengths of matriglycan. bd Microarray binding results with the matriglycan library at 100 μM utilizing b mAb IIH6 at 5 μg mL−1, c recombinantly-tagged LG4-LG5 domains of mouse Laminin α1 (His8-GFP-Lama1) at 20 μg mL−1, and d Recombinant LASV GP1 protein at 100 μg mL−1. For bd, measurements were taken at n = 4 technical replicates, where bars represent the mean and error bars represent SD. Source data of bd are provided as a Source Data file.

Back to article page