Fig. 3: Synthesis and testing of CMP-Neu5Ac matriglycan compounds for labeling of N-linked glycoproteins. | Nature Communications

Fig. 3: Synthesis and testing of CMP-Neu5Ac matriglycan compounds for labeling of N-linked glycoproteins.

From: Cell surface glycan engineering reveals that matriglycan alone can recapitulate dystroglycan binding and function

Fig. 3

a Chemoenzymatic synthesis of bi-functional CMP-Neu5Ac compounds composed of defined matriglycan polymers and a biotin functionality for protein and cell-surface glyco-engineering. Analyses of the enzymatic transfers (ET) of the synthesized compound show positive labeling of fetuin via reactivity with the anti-glyco-α-DG antibody IIH6 b, introduces the ability to bind recombinant GFP-His-Laminin LG4/5 as determined by an overlay assay c, and retards migration in SDS-PAGE as demonstrated by total protein Coomassie staining d. +Neu +Neuraminidase, +ET Enzymatic Transfer by ST6GAL1. Three independent experiments were performed with similar results each time.

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