Fig. 2: Clearance of damaged mitochondria requires key ATG components upstream of the mATG8-conjugation system. | Nature Communications

Fig. 2: Clearance of damaged mitochondria requires key ATG components upstream of the mATG8-conjugation system.

From: A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery

Fig. 2

a WT, ATG7-KO, and PINK1-KO, b TBK1-KO, c ATG9A-KO, d FIP200-KO HeLa cells stably expressing mCherry-Parkin were treated with A/O for the indicated duration. Mitochondria content was assessed by immunoblotting of proteins from different mitochondria compartments. e WT HeLa stably expressing mCherry-Parkin was treated with A/O, with or without SBI- 0206965 (25 µM). f WT and ATG7-KO cells stably expressing mCherry-Parkin were treated with A/O for 24 h, with or without Bafilomycin A1 (BafA1, 200 nM) or MG132 (10 µM) to inhibit lysosomal or proteasome degradation, respectively. g Representative immunofluorescence images of WT and ATG7-KO cells treated with A/O for 6 h and stained for HSP60 (red) and LAMP2 (green). Scale bar = 10 µm. h Quantification of A/O-treated groups in g showing percentage of LAMP2 puncta colocalised with HSP60. Mean of n = 6 fields of view ± SEM is shown. P values were calculated by two-tailed Student’s T test. *P  <  0.05, **P  <  0.01, ***P  <  0.001, and ns denotes not significant. See source data for exact P values.

Back to article page