Fig. 1: Organization and promoter consensus of the AR9 nvRNAP.
From: Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase
a Organization of the catalytically active core and promoter initiation-competent holoenzyme of the AR9 nvRNAP. A pair of genes encode a protein complex that is homologous to the bacterial subunits β or β′. The promoter specificity subunit displays no detectable sequence similarity to bacterial sigma factors. b The consensus of AR9 late promoters recognized by the nvRNAP. Both DNA strands are shown. c The dependence of the AR9 nvRNAP in vitro transcription activity on the position and number of T bases in the promoter, which is located in the template strand of DNA (bold-underlined). d The resistance of recombinantly expressed gp226 to proteolysis by trypsin. The identities and sizes of labeled major products, given as residue ranges, have been established using mass spectrometry. FL stands for the full-length protein. Two technical replicates of two biological replicates of the in vitro transcription and trypsin proteolysis experiments resulted in similar outcomes and one of them is shown. The uncropped autoradiograph and SDS PAGE are presented in Supplementary Fig. 1 and Supplementary Data 1.
