Fig. 2: High-resolution live-cell imaging of two C. albicans hyphae invading a single HeLa cell via a ‘multiple sites’ scenario and an ‘exit site’ scenario. | Nature Communications

Fig. 2: High-resolution live-cell imaging of two C. albicans hyphae invading a single HeLa cell via a ‘multiple sites’ scenario and an ‘exit site’ scenario.

From: Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage

Fig. 2

Six time points are presented, with the invasion stages defined in Fig. 1 corresponding to either filament I (‘multiple sites’ scenario) or II (‘exit site’ scenario). For each time point a phase image is presented together with a maximum intensity projection of three z-sections in the Gal-3 and CellMask (CM) channels, and a composite image showing Gal-3 (green) and CM (magenta) together. Two insets (Gal-3 and CM) are presented for each timepoint, corresponding to filament I or II. A cross-section in the CM channel through both filaments (dotted line) is presented at time point 70 min. A circular CM organization is observed around filament II but not filament I. No host cell death was observed during the course of live-cell imaging. Scale bars are 10 µm, 2 µm (inset) and 5 µm (cross-section). For other invasion scenarios see Supplementary Fig. 6.

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