Fig. 9: Cytoplasmic RNASE4 binds to and inhibits p53 in human senescent fibroblasts.
From: Senescent cells limit p53 activity via multiple mechanisms to remain viable
a Experimental approach and percentage survival of IR-senescent IMR-90 cells 6 days after knockdown of the indicated genes as assessed by MTS assay. Comparisons were made to senescent IMR-90 cells of same cultures infected with Scr-shRNA (100%). b as in a but comparisons were made to senescent IMR-90 cells of same cultures infected with Scr-shRNA or Scr- and p53-shRNA (100%). c Representative images and quantification of proximity ligation assay (PLA) to assess spatial interaction of p53 and Myc-Flag-tagged RNASE4 in indicated irradiated IMR-90 cells. Violin blot shows distribution of PLA signals per cell per IMR-90 replicate. One experiment was performed. Scale bars, 10 μm. Data represent means ± SEM. n = 4 a or n = 3 b independent experiments with each n being an average of 3 technical IMR-90 replicates, n = 3 technical IMR-90 replicates from 1 experiment in c. Statistics: one-way ANOVA with Sidak’s correction in a, b, two-tailed, unpaired t-tests in c. Source data are provided as a Source Data file.
