Fig. 5: E-cadherin overexpression rescues tissue dynamics. | Nature Communications

Fig. 5: E-cadherin overexpression rescues tissue dynamics.

From: Inhibition of negative feedback for persistent epithelial cell–cell junction contraction by p21-activated kinase 3

Fig. 5

a Mean ± S.D. of the percentages of junctions with the categorized dynamics. Bar colors correspond to Fig. 1e. Dots indicate the copy number of each E-cadherin allele. Parentheses, the number of examined junctions from n = 3 biologically independent pupae per genotype. P-values by two-tailed Dunnett’s test. b Percentages of male adult flies with genitalia angles indicated in Fig. 1b are shown. Dots indicate the copy number of each E-cadherin allele. Parentheses, the number of males examined. c A schematic illustration of Pak3-dependent junction contraction. (i)–(iv) indicate the negative feedback mechanism (green arrows, described in the “Discussion” section). Genotypes: a +/Y;E-Cad::GFP (KI)/UAS-Lifeact::Ruby;AbdB-Gal4, UAS-H2B::ECFP/UAS-Pak3 RNAi, +/Y;E-Cad::GFP (KI)/UAS-Abi RNAi;AbdB-Gal4, UAS-H2B::ECFP/UAS-Pak3 RNAi, and +/Y;E-Cad::GFP (KI)/UAS-E-Cad::GFP;AbdB-Gal4, UAS-H2B::ECFP/UAS-Pak3 RNAi; b +/Y;UAS-Abi RNAi/+;AbdB-Gal4/UAS-Pak3 RNAi and +/Y;UAS-E-Cad::GFP/+;AbdB-Gal4/UAS-Pak3 RNAi. Source data are provided as a Source data file.

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