Fig. 3: The IBIST-based optogenetic inhibition.
From: An intein-split transactivator for intersectional neural imaging and optogenetic manipulation
a Scheme depicting AAV injections into hippocampus. b Examples showing GFP fluorescence in hippocampus after injected with AAV-TRE-NpHR and AAVs (CaMKIIα promoter) of tTAN, tTAC or both, respectively. Scale bars: 500 µm. c, Summary of fluorescent intensity in b. One-way ANOVA revealed significant fluorescence differences between groups (F(2,21) = 123.1, ****P = 2.52 × 10−12) and Turkey’s multiple comparisons revealed that the fluorescence is significantly higher in tTAN+tTAC group than in others (****P < 0.0001 for all). N = 2 animals for each group. d Examples showing whole-cell current-clamp recording from hippocampal cells in brain slices upon current injections with 40 pA steps (first and second sweeps with prominent spikes; yellow with light; black without light). e Frequency of spikes in hippocampal cells in the absence (black) or presence (yellow) of 589 nm laser light (18 mW) from animals injected with AAV-TRE-NpHR and other CaMKIIα AAVs. The tTAN+tTAC animals showed a significant decrease in frequency of spikes after light stimulation (Wilcoxon matched-pairs signed rank test; first sweep, 8.3 ± 1.1 Hz vs. 1.8 ± 1.0 Hz with light; ***P = 0.001; second sweep, 17.6 ± 2.3 Hz vs. 7.1 ± 2.5 Hz, ***P = 0.001; n = 12 cells, N = 2 animals). The tTAN animals, paired t-test; first sweep, 4.5 ± 0.7 Hz vs. 4.0 ± 1.1 Hz with light, P = 0.5414; second sweep, 10.8 ± 1.6 Hz vs. 9.5 ± 2.0 Hz with light; P = 0.21; n = 10 cells, N = 2 animals. The tTAC animals, paired t-test; first sweep, 7.7 ± 1.0 Hz vs. 6.5 ± 1.2 Hz with light, *P = 0.04; second sweep, 14.8 ± 1.3 Hz vs. 14.1 ± 1.5 Hz with light, P = 0.25; n = 11 cells, N = 3 animals. Data summary: mean ± SEM. Statistical analysis: two sided. Source data are provided as a Source Data file.
