Fig. 6: Quintuple features-based Ca2+ recording.
From: An intein-split transactivator for intersectional neural imaging and optogenetic manipulation
a Scheme illustrating AAV injections to define cells by five features including quadruple retrograde tracings and pyramidal-cell-specific (CaMKIIα promoter) Ca2+ recording. b Examples showing fluorescence of GCaMP6s (green), tTAN (magenta) and blue beads (magenta). mPFC: medial prefrontal cortex. NAc: nucleus accumbens. Amy: amygdala. LS: lateral septum. Scale bars: 500 µm. c Examples of heatmaps and averaged traces of Ca2+ signals upon foot shocks between dash vertical lines. d Summary of foot shocks evoked Ca2+ responses in tTAN+tTAC (N = 6 animals) and control (1 animal for tTAN and 2 animals for tTAC) groups. First session, 1.6 ± 0.5% vs. 0.1 ± 0.06%; Mann-Whitney U test, *P = 0.0238; Second session, 0.8 ± 0.4% vs. 0.2 ± 0.1%, Mann-Whitney U test, P = 0.26. Data summary: mean ± SEM. Statistical analysis: two sided. Source data are provided as a Source Data file.
