Fig. 3: Quantitative stable-isotope tracing reveals distinct metabolic profiles in different tissues.

a Hierarchical-clustering analysis of labeled metabolites in Drosophila muscle tissues (n = 597 metabolites). b Upper panels, labeling dynamics of metabolites in each cluster; black dots represent the median labeling extent (LE) values of metabolites in the cluster in muscle tissue; black lines represent the fitted metabolic dynamics; error bands represent 95% confidence intervals. Bottom panels, enriched pathways in each cluster (p-values < 0.05; hypergeometric test). c Comparisons of labeling extents of 14 shared metabolic pathways in head and muscle tissues. d Labeling extents (LE) and labeling rates (k) of metabolites in purine metabolism (n = 10 biological replicates per group; two-tailed Student’s t-test). The centerlines of the boxplots indicate the median values; the lower and upper lines in boxplots correspond to 25th and 75th quartiles, and the whiskers indicate the largest and lowest points inside the range defined by 1st and 3rd quartile plus 1.5 times interquartile ranges (IQR). e Labeled fractions of metabolite isotopologues in purine metabolism. Bar plots represent mean ± SD (n = 10 biological replicates per group). The red dots in chemical structures represent ¹³C-labeled carbon atoms. Source data are provided as a Source Data file.