Fig. 1: A global survey identifies Lsm7 as a SG component.

a Work flow for the SG components screen under 2-DG treatment. b Fractional co-localization of GFP foci with Pab1-RFP granules based on manual fluorescence microscopic studies. Proteins that are known PB components are indicated above the gradient, and proteins that are reported as SG components (or PBs) are shown below the gradient. Previously unreported SG components are marked with an asterisk. Numbers above the gradient represent co-localization rate (%) of GFP foci with Pab1-RFP granules. Four biologically independent experiments were examined and >200 cells were analyzed for each (mean ± S.D). c Interaction network analysis for the 14 hits that co-localize with SGs together with Pab1 (colored nodes are colored based on their enrichment of Gene Ontology biological processes; gray for metabolism, pink for RNA processing; orange for protein biosynthesis, blue for stress response, brown for DNA metabolism, and red for RNA localization; gray lines indicate physical interactions, and green lines indicate genetic interactions). d Pab1-RFP granules were strongly co-localized with Lsm7-GFP foci (arrow heads indicate the co-localization). Values represent the co-localization rate (%). Scale bar indicates 2 µm. Three biologically independent experiments were examined and >200 cells were analyzed for each (mean ± S.D). e The Lsm7-GFP protein expression level was not impacted by the addition of 2-DG. Log-phase cells were treated with 400 mM 2-DG for 2 h and subsequently collected for Western blot analysis. Data are representative of three biologically independent experiments. Values are means ± S.D of the arbitrary units (intensity of target bands normalized to Pgk1 levels) for each clone. f Lsm7 proximately associates with Pab1 under 2-DG treatment. WT (BY4741) and Lsm7-5xFlag strains were grown and treated with or without 2-DG. In situ proximity, ligation assay was performed by using antibodies against Pab1 and Flag-tag. The PLA signal was assessed as described in Methods. Scale bar indicates 5 µm. Three biologically independent experiments were examined and representative images from one experiment are shown. Source data are provided as a Source Data file.