Fig. 6: Lsm7 co-phase separates with Pab1 in vitro.
From: Lsm7 phase-separated condensates trigger stress granule formation
a Purified Lsm7-GFP and Pab1-RFP phase separate in vitro with the addition of 20% Dextran 70 (pH 7.4, NaCl 150 mM, protein concentration 5 μM). Images show representative data from three independent experiments. Scale bar, 2 μm. b Lsm7-GFP and Pab1-RFP co-phase separate when added together (20% Dextran 70, pH 7.4, NaCl 150 mM, protein concentration 5 μM). Images show representative data from three independent experiments. Scale bar, 2 μm. c The addition of 0.5 µM Pab1 together with 5 µM Lsm7 results in an increased droplet formation (20% Dextran 70, pH 7.4, NaCl 150 mM). Images show representative data from three independent experiments. Scale bar, 2 μm. d Addition of total yeast RNA (200 ng/μl) enhances Lsm7-GFP droplet formation and decreases Pab1-RFP demixing (20% Dextran 70, pH 7.4, NaCl 150 mM, protein concentration 5 μM). Images show representative data from three independent experiments. Scale bar, 2 μm. e During co-phase separation of Lsm7 and Pab1 (5 μM), in the presence of total RNA (200 ng/μl), Lsm7 can rescue the demixing of Pab1 (20% Dextran 70, pH 7.4, NaCl 150 mM). Images show representative data from three independent experiments. Scale bar, 2 μm.
