Fig. 2: MNA cell survival relies on FA uptake.
From: MYCN-driven fatty acid uptake is a metabolic vulnerability in neuroblastoma
a Stable isotope tracing of FA synthesis and FA uptake in SK-N-AS MYCN-ER™ cells with or without 4-OHT (500 nM) for 24, 48, and 72 h. Mean ± SD (n = 3); two-sided unpaired t-test per time point. b Viability of NB and normal cells upon 72 h treatment with FA synthesis inhibitors (A939572, orlistat) and FA uptake inhibitors (CB16.2 and CB5). IC50 calculated in GraphPad Prism (7.01). Mean ± SD (n = 3). c Top, cell viability in complete media, delipidized media, and delipidized media supplemented with 0.025% FAs. MNA: LAN5 (0–6 day), IMR32 (0–4 day) and SK-N-BE(2c) (0–6 day); non-MNA: SHEP (0–6 day) and SK-N-AS (0–6 day). Mean ± SD (n = 3); two-way ANOVA with Dunnett’s multiple comparisons test. Bottom, Caspase 3/7 activity in complete media, delipidized media, and delipidized media supplemented with 0.025% FAs for 4 days. Mean ± SD (n = 3); one-way ANOVA with Dunnett’s multiple comparisons test. FC fold change, CM complete media, DLM delipidized media. Source data are provided in the Source Data file.
