Fig. 4: A cannabinoid biosensor with a luciferase reporter.
a The biosensor strain KM206 employs a luciferase reporter. It was incubated with a dilution series of CP55940 ranging from 100 pM to 100 nM and photographed it with a cell phone camera. This resulted in a clearly visible signal until 100 pM CP55940. b–k The response of the strain KM206 to different cannabinoids (black) was determined by incubating with a dilution series of each ligand and the resulting luminescent signal was measured using a plate reader. Control curves (gray) depict the effect of the corresponding concentrations of each ligand on the control strain KM207 activated with 1 mM adenosine. b Dose response of the strong synthetic cannabinoid CP55940. c Dose–response curve with THC, the main cannabinoid from cannabis. d Dose–response curve with 11-OH-THC, the liver metabolite of THC. e Dose–response curve of the structurally unrelated strong synthetic cannabinoid JWH-018. f–k Dose–response curves of the cannabis constituents (-)-trans-Δ9-tetrahydrocannabinolic acid (THCA), cannabichromene (CBC), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabigerol (CBG) and cannabigerolic acid (CBGA) respectively. Data presented as mean + /− standard deviation. n = 3 biologically independent samples. Source data are provided in the Source Data file.
