Fig. 2: Bioinformatic prediction of m6A contribution to stress granule enrichment. | Nature Communications

Fig. 2: Bioinformatic prediction of m6A contribution to stress granule enrichment.

From: Limited effects of m6A modification on mRNA partitioning into stress granules

Fig. 2

A Box plot of transcript length versus the number of mapped m6A sites in U-2 OS cells (Xiang et al.). Number of transcripts in each category are 3518, 3159, 2033, 970, and 712 for 0, 1, 2, 3, and 4 + m6A sites. The box plots are defined by minima, 25% percentile, median, 75% percentile, and maximum. For 0 m6A sites, the lengths are 192, 984, 1847, 3015, and 27125 for minima, 25% percentile, median, 75% percentile, and maximum, respectively. For 1 m6A sites, the lengths are 160, 1407, 2171, 3313, and 18626 for minima, 25% percentile, median, 75% percentile, and maximum, respectively. For 2 m6A sites, the lengths are 210, 1928, 2958, 4182, and 19607 for minima, 25% percentile, median, 75% percentile, and maximum, respectively. For 3 m6A sites, the lengths are 261, 2785, 4002, 5290, and 18865 for minima, 25% percentile, median, 75% percentile, and maximum, respectively. Finally, for 4 + m6A sites, the lengths are 110, 3848, 5446, 7685, and 20635 for minima, 25% percentile, median, 75% percentile, and maximum, respectively. B Scatterplot depicting predicted vs observed fraction of transcripts within stress granules. Predicted values were obtained from the linear regression model based on overall RNA length alone. C Same as B, but using a linear model based on the number of m6A sites per transcript and m6A ratio. D Same as B, but using a linear model based on both overall length, m6A sites per transcript, and m6A ratio. E Scatterplot depicting predicted fraction of transcripts within stress granules vs the number of m6A sites for RNA lengths from 500 bases to 7500 bases. F Histogram depicting the fraction of transcripts containing various m6A sites from Xiang et al. in U-2 OS cells. Source and code data are provided for this Figure (see data availability and code availability).

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