Fig. 7: hnRNPH1 is essential for the meiosis processes of early oocytes and female fertility.
From: hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells
a Co-immunofluorescence staining of hnRNPH1 and SYCP3 in ovaries from control and hnRNPH1 cKO mice at E17.5. Scale bars, 50 μm. Oocytes are indicated with white arrowheads. b Immunohistochemical staining of DDX4 in control and cKO ovaries at P1 and P3. Scale bars, 50 μm. c Co-immunofluorescence staining of SYCP3 with γH2AX in chromosome spread of pachytene oocytes from control and cKO mice at E17.5 is shown. The right histogram shows the quantification of the percentage of the cells with abnormal γH2AX signals. Scale bars, 5 μm. Data were presented as mean ± SD. A total of n = 135 control and n = 173 cKO pachytene oocytes were counted from three biologically independent mice for each genotype. A two-sided Student’s t-test was performed, **p = 0.002. d Co-staining of SYCP3 with SYCP1 in chromosome spread of pachytene oocytes from control and cKO mice at E17.5 is shown. The right histogram shows the quantification of the percentage of the cells with abnormal synapsis. Scale bars, 5 μm. Data were presented as mean ± SD. A total of n = 144 control and n = 182 cKO pachytene oocytes were counted from three biologically independent mice for each genotype. A two-sided Student’s t-test was performed, **p = 0.001. e RT-PCR analyses for indicated AS events differentially regulated genes between control and cKO ovaries at E17.5 are shown. Middle panels represent the schematic diagram of alternatively spliced exons. Right panels show the quantification of percent spliced in (PSI). Data were presented as mean ± SD, n = 3 biological replicates. A two-sided Student’s t-test was performed, top to bottom: **p = 0.0047, **p = 0.0010, **p = 0.0052, **p = 0.0030, **p = 0.0054. f–i Co-immunofluorescence staining of SYCP3 with TCF7L1 (f), TCF7L2 (g), β-catenin (h), and E-cadherin (i) in ovaries from control and cKO mice at P0. Scale bars, 50 µm. Biologically independent mice (n = 3) were examined (a, b, f–i). Source data are provided as a source data file.
