Fig. 8: hnRNPH1 cooperates with PTBP2 and SRSF3 to regulate mRNA splicing.
From: hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells
a Pie-chat shows the summary of binding locations for hnRNPH1 RIP-sequencing (RIP-seq) in spermatogenic cells isolated from P28 WT testes. b Top five sequence motifs identified from RIP-seq peaks in spermatogenic cells are shown. c Venn diagrams showing overlap of hnRNPH1 bound genes and abnormal AS genes (with ΔPSI >0.1 and ΔPSI <0.1, respectively) in hnRNPH1 cKO spermatocytes (left) and spermatids (right). d Histograms show RIP-qPCR analyses of selected mRNA of 11 genes co-precipitated by anti-hnRNPH1, anti-PTBP2, anti-SRSF3 antibodies, and control IgG in RIP experiments performed from purified germ cells. Data were presented as mean ± SD, n = 3 (three biological replicates). e RT-PCR and WB analyses of splicing assays were performed in HEK293T cells transfected with the indicated minigenes and knockdown/overexpression-related vectors for hnRNPH1, PTBP2, and SRSF3. Biologically independent experiments (n = 3) were examined. f Alternative sites in four representative genes bound directly by hnRNPH1, SRSF3, and PTBP2 from RNA-, RIP-, and CLIP-seq using IGV software. Splicing sites are indicated by the yellow box.
