Fig. 2: Most residues of the translocation pathway are critical for function. | Nature Communications

Fig. 2: Most residues of the translocation pathway are critical for function.

From: Substrate binding in the mitochondrial ADP/ATP carrier is a step-wise process guiding the structural changes in the transport cycle

Fig. 2

a Percentage of functional complementation of the WB-12 strain expressing TtAac variants compared to expressing the wild-type carrier (100% complementation), as determined by densitometry of serial dilution spot tests on YPG medium (Supplementary Fig. 2). The bars and error bars represent mean and standard deviation of four independent experiments. Significance analysis was performed with two-tailed one-sample t-tests, as described in “Methods” (p > 0.05, ns; p ≤ 0.05, *; p ≤ 0.01, **; p ≤ 0.001, ***; p ≤ 0.0001, ****). Colours represent the three observed growth properties: growth not affected significantly (ns), light blue; growth significantly affected (0.5 ≥ p > 0.0001), marine; no growth (p ≤ 0.0001), dark blue. b Position of the analysed residues in the matrix-open BKA-bound TtAac structure (PDB code: 6gci chain A), shown as a cartoon representation. Spheres represent the CA carbon atoms and are colour-coded as described in a. Source data for this figure are provided as a Source Data file.

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