Fig. 6: Patient-derived organoids evolve with time.
From: Occult polyclonality of preclinical pancreatic cancer models drives in vitro evolution
a UMAP plot of cells from a different PDO at early (green) and late (purple, navy) passages. b Pseudotime analysis performed using Monocle of single cells from early (green) and late (purple, navy) passage organoids. c Branched heatmap (left) showing dynamic gene expression along the pseudotime trajectory for each cell fate in b. Pseudotime progresses from left to right. Enriched Gene Ontology biological process terms for each gene cluster are listed on the right. d Heatmap showing inferred copy number alterations of early and late passage organoids. Cells (rows) are ordered by pseudotime. Red represents copy number gains and blue represents copy number losses. e Comparison of expression of Moffitt Basal subtype genes in scRNA of early (bottom) vs. late (top) PDO1. f Comparison of expression of Moffitt Classical subtype genes in scRNA of early (bottom) vs. late (top) PDO1.
