Fig. 1: A single-cell transcriptome atlas of 20 pig tissues.

a Schematic diagram of organs/tissues. Superscripts “c” and “n” represent the tissue analyzed by scRNA-seq and snRNA-seq respectively. OVoLT, vascular organ of lamina terminalis. b Schematic diagram of cDNA libraries generation and downstream bioinformatics analyses. The scRNA-seq and snRNA-seq were constructed independently, followed by high-throughput sequencing, and downstream bioinformatic analyses. c t-SNE visualization of all single cells in the 20 tissues. Cells are color-coded according to the tissue origin. d Pie chart showing the number of cells and proportion of cells from each tissue after filtering low-quality cells and doublets. e t-SNE visualization of all annotated major cell types from the 20 tissues. Cells are color-coded according to cell types. f Bar graph and intersect plots showing the presence of the 58 cell types across the 20 tissues. Source data are provided as a Source Data file. Schematic diagrams in a and b were created with BioRender.com.