Fig. 2: Validation of cell heterogeneity in retina and kidney. | Nature Communications

Fig. 2: Validation of cell heterogeneity in retina and kidney.

From: Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

Fig. 2

a t-SNE visualization of major cell types in the retina. b Heatmap of marker gene expression in the cell types captured in the retina. c GO term enrichment analysis on marker genes in the major cell types in the retina. The hypergeometric test was used for GO term analysis, and p values were adjusted by Benjamini & Hochberg. d t-SNE visualization of the expression of RHO, ARR3, CDHR1, GNG13, CRX, and RBP3 in the retina. e Representative IHC staining of RHO, ARR3, GNG13, CRX, CDHR1, and RBP3 in pig retina (n = 3). f t-SNE visualization of major cell types in the kidney. g Heatmap of marker gene expression in major cell types in the kidney. h GO term enrichment analysis on marker genes of major cell types in the kidney. The hypergeometric test was used for GO term analysis, and p values were adjusted by Benjamini & Hochberg. i t-SNE visualization of NPHS2, SLC12A1, PAX2, GATA2, and AQP2 expression in the kidney. j Representative IHC staining of NPHS2, SLC12A1, GATA2, AQP2, and PAX2 in pig kidney (n = 3). Source data are provided as a Source Data file.

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