Fig. 5: Lats1/2 deletion promotes melanoma formation.

a Cartoon depicting 4-HT painting experiment. b Representative image of induced Braf^V600E/Lats1/2^−/− mouse tumor, scale bar = 0.5 cm. c Representative hematoxylin and eosin staining of spontaneous or induced tumors from Braf^V600E/Lats1/2^−/− mice, scale bar = 50 µm (n > 3 spontaneous tumors, n = 2 induced tumors from Braf^V600E/Lats1/2^−/− mice). d Representative IHC of indicated proteins, scale bar = 40 µm (n > 3 spontaneous tumors, n = 2 induced tumors from Braf^V600E/Lats1/2^−/− mice). e Representative image of induced Lats1/2^−/− mouse tumor, scale bar = 0.5 cm. f Representative hematoxylin and eosin staining of induced flank tumor from Lats1/2^−/− mouse, scale bar = 50 µm (n > 6 independent mice with similar results). g Survival plot from painting to study endpoint of Lats1/2^−/− mice with 4-HT painted on flanks (Cre negative n = 4, Lats1/2^−/− n = 7, log-rank test). h Representative IHC of indicated proteins, scale bar = 40 µm (n ≥ 3 Lats1/2^−/− mice). i Cartoon depicting depilation experiment in Lats1/2^−/− mice. j Percent palpable tumorigenesis in Lats1/2^−/− mice at indicated time points (n = 10 mice, two-tailed unpaired t test). k Diameter of largest tumor on each animal measured at indicated time points (n ≥ 3 tumors measured at each timepoint with error bars ± SEM, ten mice total, two-tailed unpaired t test). Source Data are provided as a Source Data file.