Fig. 4: Adult and paediatric human GBMs show an embryonic/juvenile-like cell signature.

a Left: deconvolution analysis heatmap of malignant and non-malignant clusters from 20 adult GBM patients from Neftel et al. 2019 showing the estimated relative fraction of the mouse cell-types for each patient cluster (see Methods). The bar on the left indicates the state of the cells (malignant or non-malignant). Each patient sample was clustered separately (see patient clustering in Supplementary Fig. 5d) and the clusters are denoted as e.g., “Patient#1-cluster#1” = “Patient 1-C1” (see Supplementary Data 1). Shown on the right is a bar plot summarizing the matching results and overlap size between the patient clusters (e.g., 3 patient clusters matched to both embryonic RG and gliogenic precursors) and a schematic summary of the tumour lineage from the deconvolution analysis. b Deconvolution analysis heatmap of malignant clusters from 7 paediatric GBM patients from Neftel et al. 2019 (see Supplementary Data 1) showing the relative fraction of the mouse cell-types for each patient cluster. Each patient sample was clustered separately (see patient clustering in Supplementary Fig. 6a) and the clusters are denoted as e.g., “Patient#24-cluster#1” = “Patient 24-C1” (see Supplementary Data 1). RG Radial glia, TAPs Transient amplifying progenitors, qNSCs Quiescent neural stem cells, EEmNBs/CRCs Early embryonic neuroblasts/Cajal-Retzius cells, EEmDienNBs Early embryonic diencephalon neuroblasts, GE NBs Ganglionic eminence neuroblasts, OPCs Oligoprogenitor cells, INs Interneurons, ImStNeurons Immature striatal neurons.