Fig. 1: CMD2 type cassava varieties lose resistance upon de novo morphogenesis. | Nature Communications

Fig. 1: CMD2 type cassava varieties lose resistance upon de novo morphogenesis.

From: Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses

Fig. 1

a Left—TME204-WT CMD2-type plants challenged with cassava mosaic geminivirus remains symptom free. Middle – embryogenic structures arise from tissue culture-induced de novo morphogenesis. Right—Regenerated plant shows classic mosaic symptoms after virus challenge. b F1 populations derived from heterozygous resistant parents (NASE14, NASE19, TME14) crossed with susceptible loss-of-CMD2-resistance (LCR) line. Plants were grown and phenotyped in the field in Uganda and scored for disease over two years on a 1-5 disease score. The disease rating distribution across all populations segregates at 1:1 (χ2 p-value = 0.5263; R = 1291, S = 1259). c In the NASE14xTME204-LCR population, 125 resistant and 125 susceptible lines with consistent phenotypes over the 2 years were selected for bulk segregant analysis (BSA) mapping (solid circles).

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