Fig. 3: VIGS targeting of MePOLD1. | Nature Communications

Fig. 3: VIGS targeting of MePOLD1.

From: Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses

Fig. 3

CMD-susceptible cassava 60444 recovers from ACMV infection when MePOLD1 is downregulated by VIGS. a Percentage of symptomatic 60444 plants and (b) CMD symptom severity according to Fauquet and Fargette, 199056 18 weeks post-inoculation: ACMV (n = 15), GUS-VIGS (n = 30), MePOLD1-VIGS (n = 40), and Mock (n = 15). Bars show standard error. c Quantification of ACMV titre post-onset of CMD symptoms after inoculation with ACMV (n = 3), GUS-VIGS (n = 10), MePOLD1-VIGS (n = 10), and Mock (n = 3). Week 0 is the first onset of symptoms detected on individual plants. The data were presented as standard boxplots (the box encompasses Q1–Q3, the median is shown as a central horizontal line within the box, and the whiskers cover the data within ±1.5 IQR). Significance was determined using a two-tailed, Mann–Whitney-U test adjusted with the Benjamini–Hochberg procedure. d Representative images of the leaves used for experiments shown in panels (a–c).

Back to article page