Fig. 2: Class I crossover recombination protein HEI10 is reduced in fancm null mutants at late prophase I.

a–f Co-immunofluorescence of HEI10 (white) and ZYP1 (red) on meiotic prophase I chromosome spreads. Representative micrographs are shown from replicates, (a) (n = 82), (b) (n = 74), (c) (n = 54), (d) (n = 60), (e) (n = 54), and (f) (n = 34). Pachytene (a, c, e and f) and early diakinesis (b and d). Scale bars = 10 µm. g Counts of HEI10 foci per cell with mean values ± SD. The number of meiocytes sampled for each line is shown in brackets. *** = p < 0.001 (Mann–Whitney U Test). Source data are provided as a Source Data file.