Fig. 2: Effects of W on Wolframiiraptor (W. gerlachensis) growth in culture A10. | Nature Communications

Fig. 2: Effects of W on Wolframiiraptor (W. gerlachensis) growth in culture A10.

From: An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea

Fig. 2

a ASV abundances based on 16S rRNA gene tags from synthetic medium with and without added spring water or W. The 12 most abundant taxa are shown, which consistently made up >98% of the total ASVs. Caldimicrobium and W. gerlachensis ASV counts from triplicate cultures were statistically different between synthetic medium with and without spring water (without W addition) as denoted by the asterisk (ANOVA, *p = 0.07, **p = 0.03, n = 3). ASV counts are reported as raw reads; see Supplementary Data 9 including normalized ASV counts that show identical trends. b Absolute abundance of W. gerlachensis based on 16S rRNA gene qPCR on corn stover incubations. Central marks in boxes indicate the median, with the bottom and top edges as the 25th and 75th percentiles. Error bars denote SD of biological replicates (n = 3). c Abundance of W. gerlachensis 16S rRNA genes measured after each transfer and the resulting growth. Cultures were grown in corn stover and sugar mix in synthetic medium amended with discrete W concentrations (indicated by black arrows). 16S rRNA gene tags retained at the third transfer indicated no ASVs affiliated with W. gerlachensis in all replicates without W (Supplementary Fig. 4). Source data are provided as a Source Data file.

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