Fig. 3: 13C and 15N incorporation measured by nanoSIMS in single cells from GBS incubations cultured with isotopically labeled substrates. | Nature Communications

Fig. 3: 13C and 15N incorporation measured by nanoSIMS in single cells from GBS incubations cultured with isotopically labeled substrates.

From: An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea

Fig. 3

a Isotopic enrichments in CARD-FISH-identified W. gerlachensis (white data points) and other (black data points) cells. Central marks in boxes indicate the median, while the bottom and top edges are the 25th and 75th percentiles. Each point reflects the enrichment of a single cell. b Fluorescent (CARD-FISH) and nanoSIMS ion and ion ratio images reflecting 13C and 15N incorporation in a representative W. gerlachensis cell identified by CARD-FISH in xylose treatments (Cy3, magenta arrow). An adjacent cell (no Cy3 label, white arrow) does not exhibit 13C and 15N enrichment. The higher relative enrichment of 13C and 15N in W. gerlachensis corresponds to higher single-cell anabolic activity compared to other surrounding cells. The minimum isotope abundance ratios are set to natural abundance ratios. Source data are provided as a Source Data file.

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