Fig. 5: Tri-tissue collisions produce “escape” events.
From: Self-assembly of tessellated tissue sheets by expansion and collision
a, b Example configuration in which the magenta tissue nearly escapes in between the green tissues during expansion. Labels denote three tissues that participate in a tri-tissue collision leading to an escape event. The white line in b indicates the tissue boundary predicted by our simulation tool TissEllate based on binary collisions. Escapes occur in 50% of cases, n = 14 (Supplementary Fig. 7). c–e Close-up view of the escape process, from a different experiment with higher magnification. The same labeling scheme used as in panel a. Micrographs show one experiment, as representative example of the 7 of 14 independent experiments that resulted in escapes. f–h Tissue dynamics in a tri-tissue collision (f) feature a higher local cell speed in the escaping region than the converging region (g) with a p value of 0.006 using a two-sided Mann–Whitney U test (h). i, j A single tissue with a similar pre-escape geometry (i) does not have this speed increase in the escaping region (j). Plot shows mean with standard deviation across n = 7 (escape) and n = 8 (single tissue) independent collisions in a single experiment, of which associated micrographs are representative. Source data are provided as a Source Data file. See Supplementary Videos 11 and 12.
